| Grant Gustin | Barry Allen / The Flash |
| Candice Patton | Iris West-Allen |
| Danielle Panabaker | Caitlin Snow / Killer Frost / Frost |
| Danielle Nicolet | Cecile Horton |
| Kayla Compton | Allegra Garcia |
| Brandon McKnight | Chester P. Runk |
| Jon Cor | Mark Blaine / Chillblaine |
| Melissa Benoist | Kara Danvers / Supergirl |
| Tyler Hoechlin | Clark Kent / Superman |
| Katherine McNamara | Mia Smoak |
| Jon Cryer | Lex Luthor |
| Caity Lotz | Sara Lance / White Canary |
| Brandon Routh | Ray Palmer / The Atom / Clark Kent / Superman |
| Elizabeth Tulloch | Lois Lane |
| John Wesley Shipp | Barry Allen / The Flash (Earth 90) |
| Matt Ryan | John Constantine |
| Tom Ellis | Lucifer Morningstar |
| Stephen Lobo | Jim Corrigan / The Spectre |
| Ashley Scott | Helena Kyle / Huntress |
| LaMonica Garrett | Mar Novu / The Monitor |
| Amanda Pays | Tina McGee (archive footage) (uncredited) |
| David Harewood | J'onn J'onzz / Martian Manhunter |
| Wentworth Miller | Leonard A.I. (voice) |
| Dina Meyer | Barbara Gordon / Oracle (voice) (uncredited) |
| Ruby Rose | Kate Kane / Batwoman |
| Audrey Marie Anderson | Lyla Michaels / Harbinger |
| David Ramsey | John Diggle / Spartan |
| Stephen Amell | Oliver Queen / Green Arrow |
| Cress Williams | Jefferson Pierce / Black Lightning |
| Osric Chau | Ryan Choi |