| Vincent Martella | Phineas Flynn (voice) |
| Thomas Brodie-Sangster | Ferb Fletcher (voice) |
| Ashley Tisdale | Candace Flynn (voice) |
| Dan Povenmire | Heinz Doofenshmirtz (voice) |
| Dee Bradley Baker | Perry the Platypus (voice) |
| Alyson Stoner | Isabella Garcia-Shapiro (voice) |
| Bobby Gaylor | Buford Van Stomm (voice) |
| Maulik Pancholy | Baljeet Tjinder (voice) |
| Caroline Rhea | Linda Flynn-Fletcher (voice) |
| Richard O'Brien | Lawrence Fletcher (voice) |
| Kelly Hu | Stacy Hirano (voice) |
| Mitchel Musso | Jeremy Johnson (voice) |
| Jeff 'Swampy' Marsh | Francis Monogram (voice) |
| Tyler Alexander Mann | Carl Karl |